Roseburia intestinalis-derived FLAGELLIN (cas 12777-81-0) is a negative regulator of intestinal inflammation
-
Add time:09/04/2019 Source:sciencedirect.com
Our previous study showed that the Roseburia intestinalis (R. intestinalis), one of the dominant intestinal bacterial microbiota, was significantly decreased in Crohn's disease patients and protected colon epithelial cells from inflammatory damage. However, the roles of lncRNAs in R. intestinalis FLAGELLIN (cas 12777-81-0)-mediated anti-inflammation remain unclear. In this study, we investigate global lncRNA expression profiles using microarray analysis of ulcerative colitis samples from DSS/Flagellin-challenged mice and identified a Flagellin-induced upregulated lncRNA (HIF1A-AS2). Flagellin induced HIF1A-AS2 expression in a dose- and time-dependent manner via p38-stat1 activation. Selective pharmacological inhibitors of Stat1 and p38, and genetic knockdown of these genes abolished Flagellin-induced HIF1A-AS2 expression. In addition, luciferase reporter assay showed that Flagellin activated HIF1A-AS2 promotor via increasing stat1 phosphorylation. Silencing of HIF1A-AS2 abolished Flagellin-mediated anti-inflammatory effects, evaluating by upregulation of cytokines expression, including TNF-α, IL-1β, IL-6 and IL-12, but not TNFβ. In addition, knockdown of HIF1A-AS2 significantly increased p65 and Jnk phosphorylation, and sufficiently abolished Flagellin-mediated anti-inflammatory affects in vivo. Our study provides new insights into the mechanisms that lncRNAs regulate flagellin-mediated alleviation of colonic inflammation. It is indicated that HIF1A-AS2 may be a modulator of intestinal inflammation and represent a novel target for future therapeutics.
We also recommend Trading Suppliers and Manufacturers of FLAGELLIN (cas 12777-81-0). Pls Click Website Link as below: cas 12777-81-0 suppliers
Prev:TLR5 binding and activation by KMRC011, a FLAGELLIN (cas 12777-81-0)-derived radiation countermeasure
Next:Immunostimulatory effects of truncated and full-length FLAGELLIN (cas 12777-81-0) recombinant proteins) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Immunostimulatory effects of truncated and full-length FLAGELLIN (cas 12777-81-0) recombinant proteins09/05/2019
- TLR5 binding and activation by KMRC011, a FLAGELLIN (cas 12777-81-0)-derived radiation countermeasure09/03/2019
- RESEARCH ARTICLEMolecular cloning and functional identification of an apple FLAGELLIN (cas 12777-81-0) receptor MdFLS2 gene09/02/2019
- Toll-like receptor 5 agonist FLAGELLIN (cas 12777-81-0) reduces influenza A virus replication independently of type I interferon and interleukin 22 and improves antiviral efficacy of oseltamivir09/01/2019
- Bacteria repellent layer made of FLAGELLIN (cas 12777-81-0)08/31/2019
- Introducing a cost-effective method for purification of bioactive FLAGELLIN (cas 12777-81-0) from several flagellated gram-negative bacteria08/30/2019
- Recombinant FLAGELLIN (cas 12777-81-0)s with deletions in domains D1, D2, and D3: Characterization as novel immunoadjuvants08/29/2019
- Original ArticleAttachment of FLAGELLIN (cas 12777-81-0) enhances the immunostimulatory activity of a hemagglutinin-ferritin nano-cage08/28/2019
- OpinionBacterial FLAGELLIN (cas 12777-81-0)s: Does Size Matter?08/27/2019