Stereospecific degradation of the phenoxypropionate herbicide Dichlorprop (cas 120-36-5)
-
Add time:09/02/2019 Source:sciencedirect.com
2,4-Dichlorophenoxyacetic acid (2,4-D)/α-ketoglutarate (α-KG) dioxygenase, TfdA, from Ralstonia eutropha JMP134, was purified from recombinant cells and shown by gas chromatographic and colorimetric methods to degrade only the S enantiomer of dichlorprop, a phenoxypropionate herbicide. Similarly, cell extracts of Burkholderia cepacia RASC, containing a biochemically and genetically related α-KG-dependent dioxygenase, also were shown to oxidize (S)-dichlorprop using chiral HPLC and colorimetric methods. In contrast, cell extracts of a mecoprop-degrading strain of Alcaligenes denitrificans were shown to catabolize (R)-dichlorprop. Although the A. denitrificans activity exhibited stereospecificity opposite to that of the JMP134 and RASC strains, its cofactor requirements were found to be characteristic of an α-KG-dependent dioxygenase. A PCR amplification product from the DNA of this strain was shown to encode an amino acid sequence that was 95% and 86% identical to the corresponding region of TfdA in RASC and JMP134, respectively. Thus, closely related herbicide-degrading gene products appear to be capable of exhibiting opposite stereochemical degradative capabilities.
We also recommend Trading Suppliers and Manufacturers of Dichlorprop (cas 120-36-5). Pls Click Website Link as below: cas 120-36-5 suppliers
Prev:- Dichlorprop (cas 120-36-5)
Next:Enantioselective disturbance of chiral herbicide Dichlorprop (cas 120-36-5) to nitrogen metabolism of Arabidopsis thaliana: Regular analysis and stable isotope attempt) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Efficient enantioselective degradation of the inactive (S)-herbicide Dichlorprop (cas 120-36-5) on chiral molecular-imprinted TiO209/06/2019
- Research noteDegradation of the (R)- and (S)-enantiomers of the herbicides MCPP and Dichlorprop (cas 120-36-5) in a continuous field-injection experiment09/05/2019
- Study on the bindings of Dichlorprop (cas 120-36-5) and diquat dibromide herbicides to human serum albumin by spectroscopic methods09/04/2019
- Enantioselective disturbance of chiral herbicide Dichlorprop (cas 120-36-5) to nitrogen metabolism of Arabidopsis thaliana: Regular analysis and stable isotope attempt09/03/2019
- - Dichlorprop (cas 120-36-5)09/01/2019
- Effects of elevated ozone concentration on the degradation of Dichlorprop (cas 120-36-5) in soil08/31/2019
- Dichlorprop (cas 120-36-5) induced structural changes of LHCⅡ chiral macroaggregates associated with enantioselective toxicity to Scnedesmus obliquus08/30/2019
- Stimulation of aerobic degradation of bentazone, mecoprop and Dichlorprop (cas 120-36-5) by oxygen addition to aquifer sediment08/29/2019
- Influence of the alkyl chain length on the physicochemical properties and biological activity in a homologous series of Dichlorprop (cas 120-36-5)-based herbicidal ionic liquids08/28/2019