Rapid CommunicationInduction of cytochrome p-450 in congenic c57bl/6j mice by ISOSAFROLE (cas 120-58-1): lack of correlation with the ah locus

Add time:09/02/2019    Source:sciencedirect.com

SummaryIsosafrole induction of cytochrome P-450 was compared in congenic strains of C57BL/6J mice, one of which expresses normal levels of the Ah receptor [B6(Ahb)], and another that does not contain a measurable receptor concentration [B6(Ahd)]. Using sucrose gradient analysis of 2,3,7,8-tetrachlorodi-benzo-p-dioxin (TCDD) binding, an Ah receptor concentration of 69.1±3.8 fmol/mg protein was measured in the hepatic cytosol from B6(Ahb) mice, while no receptor could be detected in the cytosol from B6(Ahd) mice. Isosafrole treatment (75 mg/kg × 3 days) increased the total hepatic microsomal cytochrome P-450 content to the same extent in the two congenic strains. The level of microsomal monooxygenase induction in the isosafrole-treated B6(Ahd) mice was greater than that of B6(Ahb) mice for ethylmorphine N-demethylase and isosafrole metabolite-complex formation, the latter a measure of cytochrome P2-450. In the case of 7-ethoxycoumarin O-deethylase only the isosafrole-treated B6(Ahd) mice had elevated microsomal activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) also revealed a similar induction pattern for the two congenic strains, following isosafrole treatment. Thus, the isosafrole treated B6 (Ahd) mice produced an equivalent or slightly larger induction of cytochrome P-450 than the B6(Ahb) mice, suggesting that there is no direct role for the Ah receptor in the regulation of these cytochrome P-450 monooxygenase activities by isosafrole.

We also recommend Trading Suppliers and Manufacturers of ISOSAFROLE (cas 120-58-1). Pls Click Website Link as below: cas 120-58-1 suppliers

Prev:Catalytic and immunochemical properties of hepatic cytochrome P450 1A in three avian species treated with β-naphthoflavone or ISOSAFROLE (cas 120-58-1)
Next:Interactions of safrole and ISOSAFROLE (cas 120-58-1) and their metabolites with cytochromes P-450)