Regular ArticleCell-Type Specificity of l-Leucyl l-Leucine Methyl Ester

Add time:08/28/2019    Source:sciencedirect.com

l-Leucyl l-leucine methyl ester (LeuLeuOMe) is a lysosomotropic agent which is converted to a membranolytic compound by dipeptidyl peptidase I and kills human leukocytes such as CD8+ T cells and monocytes but not B cells. The reagent has also been used in mice on the assumption that the cell-type specificity to murine leukocytes is the same as that to human leukocytes. During study on the effect of LeuLeuOMe on antigen-driven IL-2 production using murine splenocytes as antigen-presenting cells, however, we noticed that murine B cells were sensitive to LeuLeuOMe. We therefore examined the cell-type specificity using murine splenocytes and peritoneal macrophages. Flow cytometric analysis revealed that the most sensitive cells to LeuLeuOMe were CD8+ cells and that CD19+ cells (B cells) were as sensitive as CD3+ cells (T cells). Murine splenic B cells, which were either positively or negatively sorted with a cell sorter, were also sensitive to LeuLeuOMe, whereas human peripheral blood B cells, which were positively sorted, were not. Peritoneal macrophages were the most insensitive to LeuLeuOMe. Thus, this study demonstrated that the cell-type specificity to murine leukocytes is different from that to human leukocytes.

We also recommend Trading Suppliers and Manufacturers of leucyl-leucine-methyl ester (cas 13022-42-9). Pls Click Website Link as below: cas 13022-42-9 suppliers

Prev:Croconic acid: An absorber in the Venus clouds?
Next:Regular ArticleSynthesis of Caged Compounds of L-Leucyl-L-Leucine Methyl Ester, an Apoptosis Inducer, and Their Cytotoxic Activity)