Structural studies on Vibrio cholerae ToxR periplasmic and cytoplasmic domains
-
Add time:09/01/2019 Source:sciencedirect.com
The transcription activator ToxR controls the expression of cholera toxin, pilus colonization factor and outer membrane protein in Vibrio cholerae. It binds to the 5′-TTTTGAT-3′ tandemly repeated DNA sequence in the cholera toxin promoter region. ToxR is a membrane protein having distinct periplasmic and cytoplasmic domains. The two domains have been cloned, over-expressed and purified for structural studies. The cytoplasmic domain is more compact than the periplasmic domain. The periplasmic domain exists as dimer due to the presence of an interchain disulfide linkage, while the cytoplasmic domain is monomeric in solution implying the importance of the disulfide bond to homodimerize the native ToxR. By replacing one of the cysteines C293 with alanine, using site-directed mutagenesis, a C293A mutant was created at the periplasmic domain to elucidate the role of cysteine in dimerization of ToxR.
We also recommend Trading Suppliers and Manufacturers of ToxR protein (cas 114265-38-2). Pls Click Website Link as below: cas 114265-38-2 suppliers
Prev:An extended ToxR POSSYCCAT system for positive and negative selection of self-interacting transmembrane domains
Next:CommunicationDimerisation of the Glycophorin A Transmembrane Segment in Membranes Probed with the ToxR Transcription Activator) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- ToxR (RegA)-mediated in vitro transcription of Pseudomonas aeruginosa toxA09/06/2019
- ArticlePeriplasmic interaction between two membrane regulatory proteins, ToxR and ToxS, results in signal transduction and transcriptional activation09/05/2019
- Detection of total and pathogenic Vibrio parahaemolyticus in shellfish: Comparison of PCR protocols using pR72H or toxR targets with a culture method09/04/2019
- ArticleCholera toxin transcriptional activator ToxR is a transmembrane DNA binding protein09/03/2019
- CommunicationDimerisation of the Glycophorin A Transmembrane Segment in Membranes Probed with the ToxR Transcription Activator09/02/2019
- An extended ToxR POSSYCCAT system for positive and negative selection of self-interacting transmembrane domains08/31/2019
- Multi-Tox: Application of the ToxR-transcriptional reporter assay to the study of multi-pass protein transmembrane domain oligomerization08/30/2019
- Changes in the periplasmic linker and in the expression level affect the activity of ToxR and λ-ToxR fusion proteins in Escherichia coli08/29/2019
- Dissection of ToxR-dependent and ToxR-independent stress-regulated pathways in Vibrio parahaemolyticus08/28/2019
-
Health and Chemical more >