Characteristics of myosin modified by β-hydroxyethyl-2,4-dinitrophenyl disulphide in the presence of pyrophosphate

Add time:09/01/2019    Source:sciencedirect.com

The influence of pyrophosphate during modification of myosin by β-hydroxyethyl-2,4-dinitrophenyl disulphide (HEDD) was studied.Pyrophosphate influenced the inhibitory phase of the action of HEDD on myosin Ca2+-ATPase (ATP phosphohydrolase, EC 3.6.1.3), and protected the ability of myosin to form the actomyosin complex. Superprecipitation of actomyosin obtained from native actin and myosin, modified in the presence of pyrophosphate, was delayed.Other HEDD-induced changes of properties of Ca2+-ATPase were not influenced by pyrophosphate. Independent of the presence or absence of pyrophosphate during modification, the Ca2+-ATPase activity of HEDD-modified myosin, in contrast to the Ca2+-ATPase activity of native myosin (1) increased with increasing pH, (2) decreased only slightly with increasing concentration of KCl, (3) was inhibited at higher concentrations of Mg2+. The actomyosin complex obtained from myosin, modified by HEDD in the presence or absence of pyrophosphate, dissociated at higher concentrations of ATP than the actomyosin obtained from native myosin and actin.

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