Magnesium in biological samples by spectrophotometric measurement of the 8-quinolinolate extract

Add time:08/29/2019    Source:sciencedirect.com

Conditions for the simple, rapid and sensitive determination of magnesium in biological samples have been determined. Commercially available solvents and reagents are used without further purification. Magnesium is separated and converted to a form which may be measured spectrophotometrically in a single extraction as the 8-quinolinolate in 1,1,2-trichloroethane. The extraction of other 8-quinolinolates is prevented by the addition of tartrate and cyanide to the buffer solution. Adequate sensitivity is obtained (absorbance of 0.4 for 20 μg of magnesium), and even greater sensitivity is possible by reducing the volumes of both aqueous and organic phases.

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