Application of a modified central composite design to optimize the capillary electrochromatographic separation of related S-oxidation compounds

Add time:08/31/2019    Source:sciencedirect.com

Capillary electrochromatography was employed to separate the antibacterial 3-[4-(methylsulfinyl) phenyl]-5S-acetamidomethyl-2-oxazolidinone from its related S-oxidation products. The separation was optimized by a systematic search for the optimal separation conditions employing a modified central composite design. The variables examined in the optimization were in terms of applied potential, volume fraction of acetonitrile and buffer (Tris hydrochloride) concentration. A response surface mapping for selected variables at an applied potential of 25 kV was also performed.

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