RESEARCH PAPERElectrogenerated Chemiluminescence Immunoassay for Human IgG with Electrochemical Polymerization-based Immobilization Method

Add time:09/06/2019    Source:sciencedirect.com

Rabbit-antihuman Immunoglobulin G (IgG) was immobilized on a gold electrode by electrochemical polymerization with pyrrole. An efficient immunoassay strategy for human IgG determined in human serum was developed based on the specific immunological reaction between the rabbit-antihuman IgG, human IgG, and Ru(bpy)32+-labled goat-anti-human IgG, which forms sandwich-typed immunocomplex. Then, the target IgG in the sandwich-typed structure was determined using electrogenerated chemiluminescence. The parameters of polymerization time, the incubation time, and the pH of detection solution were optimized. The protocol has been characterized with impendence, cyclic voltammogram, and electrogenerated chemiluminescent (ECL). A good linear relationship between ECL intensity and human IgG in the range of 0.05–2 mg l−1 was obtained, and the detection limit was 20 μg l−1.

We also recommend Trading Suppliers and Manufacturers of GOAT ANTI-RABBIT IGG; IMMOBILIZED ON (cas 111213-96-8). Pls Click Website Link as below: cas 111213-96-8 suppliers

Prev:Full length articleOriented immobilization to nanoparticles enhanced the therapeutic efficacy of antibody drugs
Next:Selective and sensitive fluorescence detection method for pig IgG based on competitive immunosensing strategy and magnetic bioseparation)