Effects of calcium on a parathyroid hormone-sensitive adenylate cyclase inhibitor

Add time:07/20/2019    Source:sciencedirect.com

Inhibition of parathyroid hormone (PTH)-sensitive adenylate cyclase by {Nle-8, Nle-18, Tyr-34} bPTH-(3–34) amide was studied in thyroparathyroid-ectomized dogs. The inhibitory effect was shown to be markedly enhanced by the addition of calcium ions into the in, vitro assay system. At 0.1 mM Ca2+, complete inhibition by the antagonist was obtained. Chelation of exogenous Ca2+ by EGTA eliminated the Ca2+-induced inhibition. Both the basal and hormone-stimulated activities were decreased in the presence of 0.1 mM Ca2+, whereas the addition of EGTA increased both activities. Our results suggest that Ca2+ modulates canine renal PTH-sensitive adenylate cyclase and its inhibition by substituted bPTH-(3–34).

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