Enhancing the catalytic efficiency of subtilisin for transesterification by dual bioimprinting

Add time:09/08/2019    Source:sciencedirect.com

Bioimprinting is a technique in which an aqueous solution of a protein molecule along with the imprint molecule is dried to remove bulk water. Subtilisin was dissolved in an aqueous buffer with a substrate analog and precipitated with the substrate alcohol to obtain a dually bioimprinted enzyme precipitate. Precipitation was found to be a better bioimprinting technique than freeze drying used so far. The precipitation also enabled simultaneous bioimprinting with the alcohol substrate reinforcing the existing imprint with the substrate analog. The dually bioimprinted subtilisin was found out to be 65-fold more efficient than the freeze dried non-imprinted powder of subtilisin and could transesterify N-acetyl-l-phenylalanine with n-propanol up to 82% in 5 h.

We also recommend Trading Suppliers and Manufacturers of SUBTILISINS (cas 1395-21-7). Pls Click Website Link as below: cas 1395-21-7 suppliers

Prev:AJM onlineReviewProprotein Convertase Subtilisin/Kexin Type 9 Inhibitors: A Brief Overview
Next:Identification of a new subtilisin-like protease NbSLP2 interacting with cytoskeletal protein septin in Microsporidia Nosema bombycis)