Protection by K+ and Mg2+ of growth and macromolecule synthesis in candicidin-treated yeast

Add time:09/09/2019    Source:sciencedirect.com

The requirements for growth and macromolecule synthesis by yeast treated with minimal lethal concentrations of the polyene antibiotic candicidin have been studied. 1.1. Glucose utilization, but not protein synthesis or growth, was protected by preincubation of cells with cofactors required for glycolysis in vitro.2.2. Preincubation with 85 mM KCl and 45 mM MgCl2 essentially eliminated the inhibition of protein synthesis and growth by candicidin. Both cations were needed during the preincubation and after addition of the antibiotic. Under these conditions amino acid uptake, RNA formation and glucose utilization were also unimpaired.3.3. Similar protection was obtained against amphotericin B or N-succinyl-fungimycin, but not against filipin. These findings emphasize the critical role of K+ and Mg2+ in the varied metabolic effects produced by the polyenes.

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