CommunicationCrystallization and preliminary characterization of mitogillin (cas 1403-99-2), a ribosomal ribonuclease from Aspergillus restrictus☆

Add time:09/03/2019    Source:sciencedirect.com

Mitogillin is a ribonuclease secreted by the fungus Aspergillus restrictus. The substrate for mitogillin is a short, universally conserved, sequence in ribosomal RNA. Cleavage of this sequence inactivates protein synthesis. Mitogillin was crystallized by a two-chamber vapor/ liquid diffusion method using ethanol as the precipitant. This method has wider potential in the use of volatile organic solvents as precipitants. Crystals of mitogillin diffract X-rays to lattice d-spacings of at least 1.6 Å, and belong to the monoclinic space group P21. with a = 50.4 Å, b = 82.4 Å, c = 38.2 Åand β = 99.8°.

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