Catabolite Gene Activator Protein

Add time:09/01/2019    Source:sciencedirect.com

Transcriptional regulation in response to the preferred sugars is a widespread phenomenon in living organisms. In Escherichia coli, carbon catabolite repression of the lactose operon is relieved in the absence of glucose in the culture medium, signaled by an increase of intracellular cyclic adenosine monophosphate (cAMP) and the activation of catabolite activator protein (CAP), also named cAMP receptor protein (CRP). The CAP protein, which has been intensively studied from the early days of molecular biology, has provided a model system for structural and mechanistic investigations on transcriptional activation in prokaryotes. CAP was the first transcriptional activator to have been purified and to have its three-dimensional structure determined, and transcription by CAP has been extensively studied, at the genetic, biochemical, and biophysical levels.

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