Analytical MethodsHigh performance liquid chromatography method for the simultaneous determination of α-, γ- and δ-tocopherol in vegetable oils in presence of hexadecyltrimethylammonium bromide/n-propanol in mobile phase

Add time:09/05/2019    Source:sciencedirect.com

A rapid methodology by RP-HPLC, 10 min of total analysis time, for the analysis of α-, γ- and δ-tocopherol in vegetable oils, in presence of retinol, retinyl acetate and α-tocopherol acetate was developed. Hexadecyltrimethylammonium bromide 0.1 M/n-propanol 65% (v/v) are used as mobile phase. Tocopherols were detected using UV and fluorescence detection. The quantification limits for α-, γ- and δ-tocopherols were 0.28, 0.12 and 0.23 mg L−1, respectively. The method yielded satisfactory results when it was applied to vegetable oils and thus, it is suitable for their quality control.

► α-, γ- and δ-Tocopherol by RP-HPLC with CTAB/n-propanol. ► Appropriate sensitivity and limits of detection and quantification values. ► Tocopherols present in vegetable oils.

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