Condensation of cytidylic acid in the presence of polyphosphoric acid

Add time:09/03/2019    Source:sciencedirect.com

1. Cytidine 2′(3′)-phosphate can be condensed in the presence of polyphosphoric acid.2. The product was found to contain polyphosphate end groups which, however, could be removed by treatment with Escherichia coli alkaline phosphatase (EC 3.1.3.1).3. A single 2′(3′)-P-terminal end group was found per chain.4. With the exception of the free P-terminal end group, there was found to be one internally bound phosphate for every cytidine residue.5. Although resistant linkages were detected, recovery of starting material from mild alkaline hydrolysis indicated that the linkages were predominantly 2′(3′)-phosphodiester in nature.6. The presence of 3′-phosphodiester linkages was demonstrated by hydrolysis with bovine pancreatic ribonuclease (EC 2.7.7.16).7. The presence of a large proportion of 5′-phosphodiester linkages was demonstrated by hydrolysis with venom phosphodiesterase (EC 3.1.4.1).

We also recommend Trading Suppliers and Manufacturers of CYCLIC CYTIDYLIC ACID SODIUM SALT (cas 15718-51-1). Pls Click Website Link as below: cas 15718-51-1 suppliers

Prev:The synthesis of 2′-homouridine, its incorporation into a dinucleoside monophosphate and hydrolytic behaviour of the dimer
Next:Reversal and deamination rates of the main ultraviolet photoproduct of cytidylic acid)