Analytical MethodsAutomatic determination of coenzyme Q10 in food using CRESYL VIOLET (cas 18472-89-4) encapsulated into magnetoliposomes

Add time:09/04/2019    Source:sciencedirect.com

A new type of magnetoliposomes (MLs), containing hydrophobic magnetic-gold nanoparticles and the long wavelength fluorophore CRESYL VIOLET (cas 18472-89-4), has been used for the determination of coenzyme Q10 (CoQ10). MLs were concentrated just before the detector, using a flow system and an external electromagnet device. The subsequent introduction of Triton X-100 and CoQ10 causes the MLs lysis and the cresyl violet oxidation, obtaining a decrease in the fluorescence signal. The dynamic range of the calibration graph was 0.03–0.50 μmol L−1 CoQ10, and the detection limit was 0.008 μmol L−1. The precision (relative standard deviation) was in the range of 1.3–4.5%. The method showed a sampling frequency of 12 h−1 and was applied to the determination of CoQ10 in several food samples. The results were compared with those obtained using a previously described chromatographic method. Also, recovery values were in the range of 83.5–101.3%.

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