Biotin and fluorescein labeling of biomolecules by active esters of 1-phenylpyrazolin-5-ones☆☆☆
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Add time:09/08/2019 Source:sciencedirect.com
The synthesis of 1-phenylpyrazolin-5-one carboxylate esters of biotin and fluorescein and their specific reactivity with amines under mild conditions to label various synthetic biopolymers such as oligonucleotides, peptides and PNA's is described.
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