Enzymatic resolution of Z-γ,γ′-di-tert-butyl-D,L-carboxyglutamic acid methyl ester

Add time:09/06/2019    Source:sciencedirect.com

A rapid and simple method for the resolution of Z-γ,γ′-di-tert-butyl-D,L-carboxyglutamic acid methyl ester is described. The new procedure is based on the enzymatic enantioselective saponification of the methyl ester by the endoprotease papain. Using a simple HPLC protocol the enantiomeric excess of Z-di-tert-butyl-L-Gla-OH has been shown to be higher than 99.5%. The procedures described in this communication allow the resolution, analysis, and downstream process of multi-gram amounts of the Gla derivative in 48 h.

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