Rapid high-performance liquid chromatographic determination of urinary N-(1-methylethyl)-N′-phenyl-1,4-benzenediamine in workers exposed to aromatic amines

Add time:09/07/2019    Source:sciencedirect.com

A procedure has been developed for determining N-(1-methylethyl)-N′-phenyl-1,4-benzenediamine in urine by using high-performance liquid chromatography. The method uses chloroform extraction for partial clean-up of the urine sample. The separation is carried out on a reversed-phase column using 65 mmol/l aqueous ammonium acetate in acetonitrile (30:70, v/v) as the mobile phase. The column effluent is monitored at 290 nm with an ultraviolet detector. The analyte is separated from other normal urine constituents in less than 4 min. Peak height and concentration are linearly related. Coefficients of variation assessed for within-day reproducibility were 5.9 and 3.7% at concentrations of 22.3 and 92.1 μg/l, respectively. The mean analytical recovery from urine samples spiked with known amounts of amine was 89.7 ± 6.8%. The request of only a small volume of urine and the simple pre-treatment procedure makes it suitable for the routine monitoring of the exposure of rubber vulcanization workers to aromatic amines.

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