Precise, direct, and rapid detection of Shigella Spa gene by a novel unmodified AuNPs-based optical genosensing system

Add time:09/25/2019    Source:sciencedirect.com

Early detection of infectious bacteria is a necessity for combating infectious diseases. Due to low infectious dose of Shigella, rapid and sensitive detection is needed. Compared to the presented genes, Spa gene can be introduced as a novel sequence for all species of Shigella detection. Herein, the possibility of Spa genes for detection of four species of Shigella was investigated for the first time by AuNPs-based optical genosensing system. In this method, AuNP–DNA probes were hybridized with Spa gene sequence. When the complementary target is present, it prevents the aggregation of the complex under acid environment and the solution remains red whereas in the absence of the specific sequence, it turns to purple. Therefore, visual detection is possible with bare eye. The comparison of this Optical DNA biosensor and PCR-based method showed that the proposed method is simple, cost-effective, rapid operation, with high or comparable detection limit of (LOD and LOQ: 8.14 and 26.6 ng mLl−1, respectively), without need of any expensive techniques, and equipments compared to the conventional methods. In conclusion, the described method may develop into a platform that could be utilized for detection of various bacterial species with high accuracy and prompt screening of samples.

We also recommend Trading Suppliers and Manufacturers of FAST RED VIOLET LB BASE (cas 121-22-2). Pls Click Website Link as below: cas 121-22-2 suppliers

Prev:One-step synthesis of carbon dots with bacterial contact-enhanced fluorescence emission: Fast Gram-type identification and selective Gram-positive bacterial inactivation
Next:A standardized protocol for comparable analysis of GSH/GSSG (cas 121-24-4) by UHPLC-ESI-MSMS for human plasma)