Capillary electrophoresis of nicotinamide—adenine dinucleotide and nicotinamide—adenine dinucleotide phosphate derivatives in coated tubular columns

Add time:09/09/2019    Source:sciencedirect.com

HPCE was shown to be an effective and convenient method for the determination of nicotinamide—adenine dinucleotide (oxidized, NAD+; reduced, NADH), nicotinamide—adenine dinucleotide phosphate (oxidized, NADP; reduced, NADPH) and their synthetic derivatives. The coenzymes were easily separated among themselves and from their degradation products, which are inhibitors of dehydrogenases, in 15 min in a coated capillary. Several coenzyme derivatives such as N6-(2-aminoethyl)-NAD(P)+ and N(1)-(2-aminoethyl)-NAD(P)+ were separated by zone electrophoresis in uncoated or coated capillaries using 50 mM 3-(N-morpholino)propanesulphonic acid (pH 7.0) or Tris—HCl (pH 8.0) as buffer systems. Capillary zone electrophoresis and micellar electrokinetic capillary chromatography can also be used to monitor continuously coenzyme chemical modifications.

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