d-Ribulose

Add time:09/24/2019    Source:sciencedirect.com

Publisher SummaryThis chapter describes the enzymatic method for the determination of D-ribulose. D-ribulose has been determined by the cysteine–carbazole method; however, this is not specific. By comparison, the high degree of specificity of the ribitol dehydrogenase from Aerobacter aerogenes allows the estimation of D-ribulose in the presence of other sugars. The enzymatic method is based on the principle that ribitol dehydrogenase catalyzes the reversible oxidation of ribitol to D-ribulose in the presence of diphosphopyridine nucleotide (DPN). The apparent equilibrium constant for this reaction is 7.17 ×10–3 at pH 8.0 and 28°C. With excess reduced diphosphopyridine nucleotide (DPNH), the D-ribulose is virtually quantitatively converted to ribitol with the simultaneous oxidation of an equivalent amount of DPNH. For the stability of the solutions, the DPNH solution should be prepared freshly each week and stored in a frozen state. The tris buffer is stable indefinitely at 4°C. The RDH solution keeps for longer than a month at 3°C. Repeated freezing and thawing leads to considerable loss of activity. The reaction proceeds stoichiometrically. For each mole of D-ribulose reduced, one mole of DPNH is oxidized to DPN.

We also recommend Trading Suppliers and Manufacturers of D-RIBULOSE 5-PHOSPHATE DISODIUM SALT (cas 108321-99-9). Pls Click Website Link as below: cas 108321-99-9 suppliers

Prev:Identification and characterization of a novel Ribose 5-phosphate isomerase B from Leishmania donovani
Next:Codon—Anticodon interaction studied with trinucleoside diphosphates containing 2-thiouridine, 4-thiouridine, 2,4-dithiouridine, or 2-thiocytidine)