Enzymatic polymerization of S4-methyl-4-thiouridine 5′-diphosphate by polynucleotide phosphorylase from Escherichia coli
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Add time:09/06/2019 Source:sciencedirect.com
ms4UDP was synthesized and found to be a substrate for polynucleotide phosphorylase (EC 2.7.7.8) from Escherichia coli having a Km value of 3.4·10−3 M. The polynucleotides poly ms4U and poly (A, ms4U1.25) showed different hyperchromic bands upon enzymatic hydrolysis to the corresponding nucleotides. Both polynucleotides exhibited emission spectra with maxima at 510 mμ and quantum efficiencies of 0.3·10−3 and 1.5·10−3 per ms4UMP residue, respectively. Some applications of the photoluminescence and absorption properties of poly ms4U are described.
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