Crosslinked poly[acryloylN,N,-bis(2,2-dimethoxyethyl) amine] and crosslinked poly[acryloylmorpholine/acryloyl N,N,-bis(2,2-dimethoxyethyl) amine] gel networks and their application to the immobilization of β-d-glucosidase

Add time:09/08/2019    Source:sciencedirect.com

The synthesis and homopolymerization of acryloyl N,N,-bis(2,2-dimethoxyethyl)amine is described. Gel networks were obtained by copolymerization of acryloyl N,N,-bis(2,2-dimethoxyethyl)amine and N,N,′-methylenediacrylamide (molar ratio 8:1) and by copolymerization of acryloyl morpholine, acryloyl N,N,-bis(2,2-dimethoxyethyl)amine and N,N,′-methylenediacrylamide (molar ratio 8:2:1). Following treatment of the gel networks with tartaric dihydrazide in dilute hydrochloric acid and subsequent activation with nitrous acid, conjugates of β-d-glucosidase were prepared. In both cases the immobilized enzyme was more resistant to heat denaturation than the native enzyme in solution. The nature of the polymer-enzyme linkage is discussed with reference to studies on the model compound phenylacetyl N,N-bis(2,2-dimethoxyethyl)amine.

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