An efficient chemical-enzymatic synthesis of 4-nitrophenyl β-xylobioside: a chromogenic substrate for xylanases

Add time:09/25/2019    Source:sciencedirect.com

4-Nitrophenyl-β-xylobioside was synthesized by an improved short chemical-enzymatic method, based on the use of xylobiose as a starting material. Xylobiose was prepared following extensive enzymatic digestion of birchwood xylan with xylanase T-6. The resulting digest, containing mainly xylobiose and xylose, was directly subjected to an acetylation step, which after silica gel chromatography, provided highly pure hexaacetate of xylobiose. Bromination with HBr in acetic acid gave in quantitative yields the corresponding bromide, which after the coupling and deprotection steps, afforded the target 4-nitrophenyl-β-xylobioside.

We also recommend Trading Suppliers and Manufacturers of 2,2-dimethyl-6-(4-nitrophenyl)-4-phenyl-1,3-diazabicyclo[3.1.0]hex-3-ene (cas 15757-54-7). Pls Click Website Link as below: cas 15757-54-7 suppliers

Prev:3a-(o-Nitrophenyl)octahydroindol-4-ones: Synthesis and spectroscopic analysis
Next:Research paperA novel compound of triphenyltin(IV) with N-tert-butoxycarbonyl-l-ornithine causes cancer cell death by inducing a p53-dependent activation of the mitochondrial pathway of apoptosis)