Steroid estrogen conjugates of hens' urine: identification of radioactive estrone-β-glucuronide, testradiol17β-3-β-glucuronide, estradiol-17α-3-β-glucuronide, estrone sulphate, estradiol-17β-3-sulphate and estradiol-17α-3-sulphate as metabolites of injected [14c]estrone

Add time:09/06/2019    Source:sciencedirect.com

[4-14C]Estrone was injected into three mature Rhode Island hens. Radioactive steroids and steroid conjugates were recovered from the urine on columns of Amberlite XAD-2 and fractionated on columns of DEAE-Sephadex A-25 in a NaCl gradient. Two minor less polar radioactive fractions (I and II) were due to free steroids. Six radioactive conjugate fractions were separated and designated III to VIII in order of increasing polarity. Fraction III was identified as estrone-β-glucuronide; Fraction IV as estradiol-17β-3-β-glucuronide plus estradiol-17α-3-β-glucuronide; Fraction V as estrone sulphate; and Fraction VII as estradiol-17β-3-sulphate plus estradiol-17α-3-sulphate. Fraction VI was identified as a monosulphate. Fraction VIII was not identified definitively.

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