Isolation, partial chemical and biological characterization of thymone B

Add time:07/16/2019    Source:sciencedirect.com

A peptide, thymone B, was isolated from bovine thymus by sequences of solvent extractions, gel filtrations, ion exchange chromatography and micromanipulation. Paucity of sample restricted all assays. Thymone B showed essentially single spots by TLC in 3 systems and by electrophoresis in one system. Levels of 100 nanograms (lower?) and 10 micrograms (lowers?) of thymone B stimulated incorporation of [3H]-thymidine into DNA, and the synthesis of cGMP, respectively; 100 micrograms did not stimulate cAMP. Trypsin destroyed activity indicating an active peptide. Analysis indicated up to 13 individual amino acids: Asp, Glu, Gly, Ala, Val, Ile, Leu, Ser, Pro, Thr, His, Lys and Arg, and a molecule considerably smaller than that of thymone A.

We also recommend Trading Suppliers and Manufacturers of thymone C (cas 76775-50-3). Pls Click Website Link as below: cas 76775-50-3 suppliers

Prev:The finding and partial purification and characterization of thymone C (cas 76775-50-3)
Next:Isolation of thymone A from bovine thymus partial chemical and biological characterization)