Alterations in Ascorbic Acid Levels by Transformation of the L-GALACTONO-1,4-LACTONE (cas 1668-08-2) Dehydrogenase Gene in Solanum tuberosum
-
Add time:09/07/2019 Source:sciencedirect.com
L-galactono-1, 4-lactone dehydrogenase (GLDH) is an important enzyme that catalyzes the last step of the ascorbate biosynthetic pathways in plants. A full-length cDNA clone encoding GLDH was isolated from potato (Solanum tuberosum L. ‘Favorita’) leaf and subcloned into a binary vector, pBI121, to construct sense and antisense recombinant plant expression vectors. The recombinants were introduced into potato via Agrobacterium-mediated transformation, and plants were confirmed as transgenic using PCR and quantitative real-time PCR. Two anti-sense potato lines (G1 and G2) and three sense lines (G3, G4 and G5) were obtained. The GLDH activity of G4 and G5 were increased in vivo. Moreover, the ascorbic acid (AsA) and dehydroascorbate (DHA) contents were up-regulated in both leaves and tubers. However, the shoots of G1 were suppressed and its leaves were deformed. Additionally, the AsA contents in G1 leaves and tubers decreased by 28.8% and 10.3%, respectively. The GLDH activity in leaves treated with L-GALACTONO-1,4-LACTONE (cas 1668-08-2) (L-GalL) increased in all lines, as did the AsA and DHA contents. These results indicate that GLDH activity plays an important role in regulating the AsA level as well as the growth and development of potato plants.
We also recommend Trading Suppliers and Manufacturers of L-GALACTONO-1,4-LACTONE (cas 1668-08-2). Pls Click Website Link as below: cas 1668-08-2 suppliers
Prev:Theoretical investigation of conformational stabilities and 13C NMR chemical shifts of a seven-membered ring thiosugar, (3R,4R,5R,7S)-7-(hydroxymethyl)thiepane-3,4,5-triol
Next:PhysiologyTiller number is altered in the ascorbic acid-deficient rice suppressed for L-GALACTONO-1,4-LACTONE (cas 1668-08-2) dehydrogenase) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Research paperCharacterization of the galactono-1,4-lactone dehydrogenase from pepper fruits and its modulation in the ascorbate biosynthesis. Role of nitric oxide☆09/28/2019
- Conformationally constrained analogues of diacylglycerol. 5. 2,5-Dideoxy-3-O-tetradecanoyl-D-galactono-1,4-lactone: A superior homologue of 3-O-tetradecanoyl-2-deoxy-L-ribonolactone with PK-C binding affinity.☆10/01/2019
- Standard molar enthalpies of formation of crystalline stereoisomers of aldono-1,4-lactones09/27/2019
- Functional assignment of Glu386 and Arg388 in the active site of l-galactono-γ-lactone dehydrogenase09/26/2019
- Ascorbic acid in pollen: Conversion of L-GALACTONO-1,4-LACTONE (cas 1668-08-2) to L-ascorbic acid by Lilium longiflorum☆09/25/2019
- Liquid crystalline 5,6-O-acetals of L-GALACTONO-1,4-LACTONE (cas 1668-08-2) prepared by a microwave irradiation on montmorillonite09/24/2019
- An efficient three step synthesis of vitamin C from L-GALACTONO-1,4-LACTONE (cas 1668-08-2), a by-product of the sugar industry09/10/2019
- l-Ascorbate biosynthesis in strawberries: L-GALACTONO-1,4-LACTONE (cas 1668-08-2) dehydrogenase expression during fruit development and ripening09/09/2019
- PhysiologyTiller number is altered in the ascorbic acid-deficient rice suppressed for L-GALACTONO-1,4-LACTONE (cas 1668-08-2) dehydrogenase09/08/2019