Enantiomeric and diastereomeric high-performance liquid chromatographic separation of cyclic β-substituted α-amino acids on a teicoplanin chiral stationary phase

Add time:09/07/2019    Source:sciencedirect.com

High-performance liquid chromatographic (HPLC) separation of stereomeric cyclic β-substituted α-quaternary α-amino acids was performed on a chiral stationary phase based on the glycopeptide antibiotic teicoplanin. The investigated amino acids are the 1-amino-2-methylcyclohexanecarboxylic acids, the 1-amino-2-hydroxycyclohexanecarboxylic acids, Ala, Cha, Phe and Tle. The effects of the mobile phase composition (type and content of organic modifier, pH) and of the temperature on the enantio- and diastereoselectivity were studied and the conditions were optimised to resolve the four stereomers of one amino acid in a single chromatographic run. The influence of the modifier concentration and the pH of the mobile phase reveal two enantiomeric and diastereomeric discrimination mechanisms based on different interactions with the stationary phase. For optimal separation of diastereomers the column has to be conditioned with an acidic eluent.

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