Quantitation of N,N,N′,N′-tetrakis(2-hydroxypropyl)-ethylenediamine in plasma by gas chromatography

Add time:09/10/2019    Source:sciencedirect.com

A wide-bore capillary gas chromatographic method with nitrogen-selective thermionic detection is described for the quantitative analysis of N,N,N′,N′-tetrakis(2-hydroxypropyl)ethylenediamine (Quadrol) in plasma. N,N,N′,N′-tetrakis(2-hydroxybutyl)ethylenediamine is used as an internal standard. Rat or human plasma samples (0.5 ml) are mixed with internal standard, adjusted to alkaline pH and subjected to a single extraction with dichloromethane. Quadrol recovery from plasma typically exceeds 90%. The method is linear over the range 1.0–50 μg/ml. The working detection limit is 0.5 μg/ml and the analysis time is under 7 min. The procedure has been used to obtain plasma concentration versus time data for the evaluation of Quadrol pharmacokinetics in rats.

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