Interaction of prednisone with dsDNA at silver nanoparticles/poly(glyoxal-bis(2-hydroxyanil))/dsDNA modified electrode and its analytical application

Add time:09/25/2019    Source:sciencedirect.com

This paper reports the fabrication of an electrochemical DNA biosensor for the electrochemical determination of prednisone (PRD), which is a synthetic corticosteroid. For this purpose, silver nanoparticles (AgNPs) and a new polymer film poly(glyoxal-bis(2-hydroxyanil)) (P(GBHA)) were electrochemically deposited on a glassy carbon electrode (GCE), respectively. Then, an electrochemical DNA biosensor was prepared onto this electrode surface (GCE/AgNPs/P(GBHA)) by the immobilization of dsDNA using a chronoamperometry method. The proposed electrode was characterized by FESEM, XPS, and cyclic voltammetry (CV). The interaction between the PRD and dsDNA immobilized on the GCE/AgNPs/P(GBHA) electrode was investigated via a differential pulse voltammetry (DPV) method and UV–Vis spectrophotometry. The experimental factors affecting the interaction between the PRD concentration and dsDNA were optimized. The fabricated biosensor showed a wide linear response in a PRD concentration range of 1.0–50.0 μg mL−1 depending on both the adenine and guanine base signals. The detection limit based on the guanine and adenine signals was 0.3 μg mL−1 and 0.25 μg mL−1, respectively. The sensor exhibited excellent anti-interferential ability, good stability and reproducibility and was satisfactorily employed for the electrochemical assay of PRD in serum samples. The new DNA biosensor can be utilized for the sensitive, accurate and rapid analysis of PRD.

We also recommend Trading Suppliers and Manufacturers of Prednisone 21-acetate (cas 125-10-0). Pls Click Website Link as below: cas 125-10-0 suppliers

Prev:ArticlesAddition of radium-223 to abiraterone acetate and prednisone or prednisolone in patients with castration-resistant prostate cancer and bone metastases (ERA 223): a randomised, double-blind, placebo-controlled, phase 3 trial
Next:Research PaperBiotransformation of prednisone and dexamethasone by cytochrome P450 based systems – Identification of new potential drug candidates)