Gas chromatographic determination of urinary methylmalonic acid

Add time:07/16/2019    Source:sciencedirect.com

A method for the determination of methylmalonic acid in urine has been developed. The method consists of essentially two operations: (1) A separation of methylmalonic acid from most of the urinary components by ion-exchange chromatography. (2) A gas chromatographic determination of methylmalonic acid by the technique of decarboxylation and chromatography of the monocarboxylic acid formed. Other malonic acids are used as internal standards in the gas chromatographic analysis.

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