Analysis of S-METHYLMETHIONINE (cas 13065-25-3) and S-adenosylmethionine in plant tissue by a dansylation, dual-isotope method
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Add time:09/27/2019 Source:sciencedirect.com
A method is presented for determining the levels of S-METHYLMETHIONINE (cas 13065-25-3) (MeMet) and S-adenosylmethionine (AdoMet) in the same plant tissue sample, utilizing readily available equipment. The bottom limit of sensitivity, ca. 100 pmol, can be lowered if required. A trichloracetic acid homogenate of the tissue is supplemented with [carboxyl-14C]MeMet and [carboxyl-14C]AdoMet. After separation of MeMet and AdoMet from each other and from endogenous homoserine on a phosphocellulose column, the two fractions are heat treated at appropriate pH values to liberate [14C]homoserine. Quantitation is via the 3H14> ratio of [3H]dansyl-[14C]homoserine isolated by thin-layer chromatography. The method is validated with pea cotyledon, corn root, and cauliflower leaf.
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