1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide interference with Lowry method

Add time:09/10/2019    Source:sciencedirect.com

1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was tested for interference with the Lowry method of protein determination. The EDC interference was nearly additive at both 660 and 750 nm. Blue-colored complex developed in the case of EDC and showed maximum absorbance at 750 nm, similar to the bovine serum albumin (BSA) estimation. In time response analysis, blue-colored complex was developed after 30 min of incubation in both cases (EDC and BSA); therefore, it followed the same kinetic pattern of color development as that of BSA. Interference is believed to be caused by the reduction of the folin–ciocalteu reagent by EDC, resulting in increased blue-colored complex and apparently causing increased protein content of the sample.

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