Identification of Dimedone (cas 126-81-8)-trapped sulfenylated proteins in plants under stress
-
Add time:09/10/2019 Source:sciencedirect.com
In stressed plants, the reactive oxygen species (ROS) levels rise. Key to ROS signaling research are detection and identification of the protein cysteine sulfenylation (-SOH), the ROS-mediated oxidative product of a thiol (-SH). Arabidopsis thaliana seedlings were stressed with hydrogen peroxide (H2O2) and the sulfenylated proteins were tagged with dimedone. Dimedone-tagged sulfenic acid proteins were visualized on a two-dimensional electrophoresis (2DE) immunoblot with an anticysteine sulfenic acid antibody and were subsequently detected by mass spectrometry. We optimized the detection method for protein sulfenylation in Arabidopsis. We conclude that dimedone can penetrate the cell wall, does not stress plants, and can “read” the changes in the protein sulfenylation pattern under oxidative stress. We observed that the number of sulfenylated proteins in plants treated with 10 mM H2O2 was higher than that in untreated plants. A total of 39 sulfenylated protein spots were found on 2DE immunoblots. By means of mass spectrometry, 11 sulfenylated proteins were discovered involved in primary metabolism, redox regulation, translation and signaling pathways. Hence, by combining an immunochemical 2DE strategy with mass spectrometry, we were able to identify sulfenylated proteins in H2O2-stressed Arabidopsis seedlings. The sulfenylated proteins can be considered for further validation as redox regulators in plants.
We also recommend Trading Suppliers and Manufacturers of Dimedone (cas 126-81-8). Pls Click Website Link as below: cas 126-81-8 suppliers
Prev:Original articleA concise study on Dimedone (cas 126-81-8): A versatile molecule in multi-component reactions, an outlook to the green reaction media
Next:Original articleTrivalent transition metal complexes derived from carbohydrazide and Dimedone (cas 126-81-8)) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Non-catalytic solvent-free synthesis of 5,6,7,8-tetrahydro-4H-chromenes from aldehydes, Dimedone (cas 126-81-8) and malononitrlie at ambient temperature09/30/2019
- Generation of pyrrolo[2,3-d]pyrimidines. Unexpected products in the multicomponent reaction of 6-aminopyrimidines, Dimedone (cas 126-81-8), and arylglyoxal09/29/2019
- Strong intramolecular hydrogen bonding within the Dimedone (cas 126-81-8)–aldehyde adducts09/28/2019
- Highly selective detection of disulfenylated proteins through a Dimedone (cas 126-81-8)-based fluorescent probe and application in cells10/01/2019
- Synthesis and in vitro evaluation of 2-(((2-ether)amino)methylene)-Dimedone (cas 126-81-8) derivatives as potential antimicrobial agents09/27/2019
- Original articleTandem Knoevenagel–Michael reactions in aqueous diethylamine medium: A greener and efficient approach toward bis-Dimedone (cas 126-81-8) derivatives09/26/2019
- Highly enantioselective Michael/cyclization tandem reaction between Dimedone (cas 126-81-8) and isatylidene malononitriles09/25/2019
- Original articleTrivalent transition metal complexes derived from carbohydrazide and Dimedone (cas 126-81-8)09/24/2019
- Original articleA concise study on Dimedone (cas 126-81-8): A versatile molecule in multi-component reactions, an outlook to the green reaction media09/09/2019