Analytical studies on the chiral separation and simultaneous determination of pantothenic acid and Hopantenic (cas 18679-90-8) acid enantiomers in rat plasma by gas chromatography—mass fragmentography

Add time:09/24/2019    Source:sciencedirect.com

The chiral separation and simultaneous determination of d- and l-pantothenic acids and d- and l-Hopantenic (cas 18679-90-8) acids in rat plasma using gas chromatography—mass fragmentography are described. The method is based on deproteinization by ion-exchange resin, extraction with ethyl acetate under acidic conditions, and derivatization to form several interesting compounds. After methyl esterification for carboxylic acid of d- and l-pantothenic acids, d- and l-hopantenic acids could be derivatized to trifluoroacetate, cyclic sulphite, and cyclic n-butylboronate for hydroxy groups. d- and l-forms of these derivatives were completely separated by mass fragmentographic technique with quasi-molecular ions. Calcium salt of d-5-[(2,4-dihydroxy-3,3-dimethyl-1-oxobutyl)amino]pentanoic acid was used as an internal standard for the determination of dl-pantothenic acids and dl-hopantenic acids. The detection limits of dl-pantothenic acids and dl-hopantenic acids in this method were 5 and 12 ng, respectively. This method could be applied to the study of plasma levels of d-, l-pantothenic acids and d-, l-hopantenic acids in rat.

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