Organic acids and stable isotope metabolic studies of a thermophilic sulfur-dependent anaerobic archaeon

Add time:09/24/2019    Source:sciencedirect.com

Organic acids were analyzed as tert-butyldimethylsiyl derivatives by gas chromatography and gas chromatography-mass spectrometry in cultures of an extremely thermophilic sulfur-dependent anaerobic archaeon. strain ST 546, isolated from a deep-sea hydrothermal vent. Quantitative data indicate the formation of propanoic, 2-methylpropanoic, 3-(methylthio)propanoic, phenylacetic, 4-hydroxyphenylacetic and indole-3-acetic acids (all detected at the millimolar level) and one compound tentatively identified to 2- and/or 3 methylbutanoic acid in peptone media. In addition to these compounds, formic and 2-hyroxypropanoic acids (all the acids detected at the sub-millimolar level) along with another compound are associated with the 20 amino-acid containing medium culture. Use of stable-isotope labelled amino acids demonstrated that 3-(methylthio)propanoic acid originates from L-methionine whereas L-phenylalanine yields phenylacetic acid, but not 4-hydroxyphenylacetic acid.

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