Sodium Saccharin Inhibits Adenylyl Cyclase Activity in Non-Taste Cells

Add time:09/30/2019    Source:infona.pl

We have studied the in vitro effect of sodium saccharin (NaSacch) on the rat adipocyte adenylyl cyclase complex. NaSacch (2.5-50 mM) inhibited significantly in a dose-dependent manner basal and isoproterenol-stimulated cAMP accumulation on isolated rat adipocytes. Similarly, NaSacch (2.5-50 mM) inhibited forskolin-stimulated adenylyl cyclase activity measured in the presence of Mg 2 + -ATP on adipocyte, astrocyte and thyrocyte membrane fractions. In contrast, NaSacch did not inhibit but slightly increased the forskolin-stimulated adenylyl cyclase activity measured in the presence of Mn 2 + -ATP and GDPβS, a stable GDP analogue. The effect of NaSacch was not mediated through either the A 1 -adenosine receptor (A 1 R) or the α 2 -adrenergic receptor (α 2 AR). The inhibitory effect of NaSacch was additive to that of A 1 R agonist and was not blocked by the addition of the α 2 AR antagonist RX 821002. Pretreatment of adipocytes with pertussis toxin slightly attenuated but did not abolish the inhibitory effect of NaSacch on forskolin-stimulated adenylyl cyclase activity on membrane fractions. These data suggest that the inhibitory effect of NaSacch on forskolin stimulated-adenylyl cyclase in adipocytes does not imply only Gi protein but also other direct or indirect inhibitory pathway(s) which remain to be determined.

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