Determination of viridicatin (cas 129-24-8) inPenicillium cyclopiumby Capillary Gas Chromatography

Add time:09/28/2019    Source:infona.pl

A sensitive gas chromatographic method for the determination of the quinoline alkaloid viridicatin (cas 129-24-8), a fungal metabolite isolated from severalPenicilliumspecies and presumably a biogenetic precursor to naturally occurring diazepam-like 1,4-benzo-diazepines, has been developed. Prior to conversion of viridicatin into a volatile trimethylsilyl derivative, a rapid sample clean-up was accomplished by solid-phase extraction from mould mycelium on a C18cartridge. Using synthetical 6-bromo-viridicatin as an internal standard, the amount of viridicatin producedde novoin emerged grown cultures ofPenicillium cyclopiumstrain SM 72 was calculated in correlation to the duration of mould cultivation.

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