Protein kinase C in focal ischemic rat brain: dual autoradiographic analysis of [ 1 4 C]IODOANTIPYRINE (cas 129-81-7) (IAP) and [ 3 H]phorbol-12,13-dibutyrate (PDBu)

Add time:09/26/2019    Source:infona.pl

Protein kinase C (PKC) activity was measured in rat brain with 2 h of middle cerebral artery (MCA) and common carotid artery (CCA) occlusion, using dual autoradiography of [ 1 4 C]iodoantipyrine (IAP) and [ 3 H]phorbol-12,13-dibutyrate (PDBu). In the ischemic brain, it required more than 120 min of incubation to obtain a plateau in PDBu binding. In contrast, the binding of PDBu in non-ischemic brain reached a plateau with incubation for 60 min. This delay of PDBu binding in the ischemic brain suggests that the affinity of this ligand is reduced due to a change in structure of the cell membrane caused by ischemia. PDBu binding in the ischemic brain increased significantly compared to the non-ischemic brain. This finding provides further evidence that excessive activation of PKC in the ischemic brain may play an important role in ischemic neuronal damage. (C)1997 Elsevier Science B.V. All rights reserved.

We also recommend Trading Suppliers and Manufacturers of IODOANTIPYRINE (cas 129-81-7). Pls Click Website Link as below: cas 129-81-7 suppliers

Prev:A [ 14 C]IODOANTIPYRINE (cas 129-81-7) study of inter-regional correlations of neural substrates following central post-stroke pain in rats
Next:Local hemodynamic changes during transient middle cerebral artery occlusion and recirculation in the rat: a [ 1 4 C]IODOANTIPYRINE (cas 129-81-7) autoradiographic study)