Three-dimensional structure of ribonuclease Ms*3'-guanylic acid complex at 2.5 Å resolution

Add time:09/26/2019    Source:infona.pl

The crystal structure of ribonuclease Ms*3 + -guanylic acid complex has been determined by molecular replacement methods based on the known structure of ribonuclease T 1 . The pattern of hydrogen-bonds between the enzyme and the guanine base is similar to that discovered by Arni et al. [(1988) J. Biol. Chem. 263, 15358-15368] in the crystal structure of ribonuclease T 1 *2'-guanylic acid complex. As for the possible general base in the trans-phosphorylation step of the catalysis, 0 c 1 of Glu 1 7 is within the hydrogen-bond distance (2.7 Å) of the 2'-0 of the nucleotide while N c 2 of His 1 4 is significantly more distant (3.4 Å) from the 2'-0.

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