Production of 2-Methyl-1-butanol (cas 137-32-6) in engineered Escherichia coli
-
Add time:09/30/2019 Source:infona.pl
Recent progress has been made in the production of higher alcohols by harnessing the power of natural amino acid biosynthetic pathways. Here, we describe the first strain of Escherichia coli developed to produce the higher alcohol and potential new biofuel 2-Methyl-1-butanol (cas 137-32-6) (2MB). To accomplish this, we explored the biodiversity of enzymes catalyzing key parts of the isoleucine biosynthetic pathway, finding that AHAS II (ilvGM) from Salmonella typhimurium and threonine deaminase (ilvA) from Corynebacterium glutamicum improve 2MB production the most. Overexpression of the native threonine biosynthetic operon (thrABC) on plasmid without the native transcription regulation also improved 2MB production in E. coli. Finally, we knocked out competing pathways upstream of threonine production (ΔmetA, Δtdh) to increase its availability for further improvement of 2MB production. This work led to a strain of E. coli that produces 1.25 g/L 2MB in 24 h, a total alcohol content of 3 g/L, and with yields of up to 0.17 g 2MB/g glucose.
We also recommend Trading Suppliers and Manufacturers of 2-Methyl-1-butanol (cas 137-32-6). Pls Click Website Link as below: cas 137-32-6 suppliers
Prev:Preparation of helical mesoporous ethylene–silica nanofibers using CTAB and (S)-2-Methyl-1-butanol (cas 137-32-6)
Next:Conductance Studies in Mixtures of Water with DMF at 298.15 K. Part VI. Lithium and Sodium Nitrates, Sodium Perchlorate and Propionate, Potassium Picrate and Thiocyanate, and Limiting Ionic Conductance) - 【Back】【Close 】【Print】【Add to favorite 】
- Related Information
- Preparation of helical mesoporous ethylene–silica nanofibers using CTAB and (S)-2-Methyl-1-butanol (cas 137-32-6)09/29/2019
- Volumetric and ultrasonic study of mixtures of 2-phenylethanol with 1-butanol, 2-butanol, and 2-Methyl-1-butanol (cas 137-32-6) at T=(298.15–323.15) K and atmospheric pressure: Measurement and prediction10/01/2019