Activation of 2-Aminofluorene (cas 153-78-6) by Prostaglandin Endoperoxide H Synthase 2

Add time:09/29/2019    Source:infona.pl

Prostaglandin endoperoxide H synthase is the key enzyme in the conversion of arachidonic acid to tissue prostanoids. Two isoforms of prostaglandin endoperoxide H synthase have been identified: PHS-1 is constitutively expressed in most tissues under normal physiological conditions and PHS-2 is expressed in response to inflammatory agents, tumor promoters, and other agents related to mitogenesis. Previous work demonstrated that PHS-1 can activate arylamine carcinogens. We report here that PHS-2 can also activate an arylamine carcinogen to form DNA adducts. This is shown by: (1) use of purified ovine PHS-2 to form DNA adducts; (2) increased DNA adduct formation, PHS-2 mRNA, and PHS-2 protein after treatment of HUVEC cells with the PHS-2 inducer PMA; and (3) transient expression of PHS-2 cDNA in COS-1 cells gave rise to both elevations of PHS-2 enzyme protein and DNA adduct formation. Finally, two PHS inhibitors, aspirin and indomethacin, showed significant inhibition of PHS-2-mediated DNA adduct formation.

We also recommend Trading Suppliers and Manufacturers of 2-Aminofluorene (cas 153-78-6). Pls Click Website Link as below: cas 153-78-6 suppliers

Prev:Arylamine N-Acetyltransferases: Covalent Modification and Inactivation of Hamster NAT1 by Bromoacetamido Derivatives of Aniline and 2-Aminofluorene (cas 153-78-6)
Next:First Principles Study of Linear and Nonlinear Optical Properties of 2-Aminofluorene (cas 153-78-6) (C13H11N))