Biosynthesis of pure araban (cas 11078-27-6) and xylan

Add time:07/18/2019    Source:sciencedirect.com

A crude particulate enzyme preparation from mung bean shoots partially freed from sugar transferases synthesized pure araban (cas 11078-27-6) from UDP-l-14C-arabinose. The preparation thus allowed to study some properties of the UDP-arabinose transferase which was shown to require 7 mM Mn2+ and pH 6–6·5 for optimal activity. Pure xylan was synthesized from UDP-d-14C-xylose if a mixture of 0·06% Triton X100 and 35 mM EDTA was added to the crude enzyme preparation. In contrast to the UDP-arabinose transferase the UDP-xylose transferase does not require bivalent metal ions.

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