Stereoselectivity in the elution of sugars from columns of SEPHADEX G-15 (cas 11081-40-6)

Add time:07/19/2019    Source:sciencedirect.com

The gel-permeation characteristics of selected sugars and sugar derivatives were determined on a column of Sephadex G-15. The following observations were made: (1) the Sephadex G-15 column does not distinguish between enantiomeric saccharides, (2) a decrease in Rg, value is observed on going from a methylene (deoxy) to a hydroxyl to a methoxyl group in monosaccharides, (3) methylation or reduction of a particular hydroxyl group affects the Rg, values selectively, (4) the substituted (methyl or glucosyl) α anomer is retained on the column longer than the corresponding β anomer, and (5) sugars having either one or no axially attached hydroxyl group are eluted in the order (increasing Rg value): axially attached hydroxyl groups at C-4, at no carbon atom, at C-2, and at C-3.

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