Radioimmunoassay of 5-methyl-2′-deoxycytidine. A method for the quantitation of DNA methylation
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Add time:07/18/2019 Source:sciencedirect.com
5-Methyl-2′-deoxycytidine (5MedCyd) is a minor constituent of mammalian cell DNA. We report the production and characterization of highly specific rabbit anti-5MedCyd antiserum. The antiserum was suitable for the radioimmunological measurement of 5MedCyd. This simple radioimmunoassay was capable of quantitating calf thymus DNA methylation at nanomolar levels of total DNA.
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