The radiometric assay of alkaline phosphatase activity with 125I-labelled phenolphthalein monophosphate (cas 13306-67-7)

Add time:07/17/2019    Source:sciencedirect.com

The coupling of 125I to phenolphthalein monophosphate is described. Hydrolytic cleavage of the phosphate group by alkaline phosphatases (EC 3.1.-3.1) releases labelled phenolphthalein which is extracted into ethyl acetate at pH 10. This forms the basis of a sensitive assay for the enzyme with the advantages of sensitivity and rapidity from the use of a γ-emitter. Results with Escherichia coli and calf intestine alkaline phosphatases are presented.

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