Molecular cloning and expression of farnesyl pyrophosphate synthase gene responsible for essential oil biosynthesis in hop (Humulus lupulus)

Add time:07/18/2019    Source:sciencedirect.com

SummaryFarnesyl pyrophosphate (FPP) synthase catalyzes the biosynthesis of geranyl pyrophosphate (GPP) and FPP, which are the precursors of terpenoids such as essential oils, from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). gDNA and cDNA encoding hop (Humulus lupulus L.) FPP synthase was cloned by the Inverse PCR method and the Cassette-mediated PCR method and sequenced. The deduced amino acid sequence showed a high identity to plant FPP synthases. The cloned gene was expressed in Escherichia coli cells to produce the FPP synthase. Northern blot analysis revealed that the FPP synthase gene was expressed in the lupulin gland, bract, leaf, and stem of hop and expressed more strongly in the lupulin gland than in other tissues.

We also recommend Trading Suppliers and Manufacturers of ISOPENTENYL PYROPHOSPHATE TRIAMMONIUM SALT SOLUTION (cas 116057-53-5). Pls Click Website Link as below: cas 116057-53-5 suppliers

Prev:The inhibition of human farnesyl pyrophosphate synthase by nitrogen-containing bisphosphonates. Elucidating the role of active site threonine 201 and tyrosine 204 residues using enzyme mutants
Next:The biosynthesis of lubimin from [1-14C]isopentenyl pyrophosphate by cell-free extracts of potato tuber tissue inoculated with an elicitor preparation from Phytophthora infestans)