Detail of "56-82-6"

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Insulin release from a cloned precursor beta cell line

Insulin release from a cloned precursor beta cell line. Ng, K. W.; Gummer, P. R.; Grills, B. L.; Michelangeli, V. P.; Dunlop, M. E. (Repatriat. Gen. 816-66-0 and 9035-68-1 are cas registry numbers. These chemicals are also mentioned in this article. Hosp., Univ. Melbourne, Heidelberg 3081, Australia). J. Endocrinol., 113(1), 3-10, 3 plate (English) 1987. CODEN: JOENAK. ISSN: 0022-0795. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) The establishment in long-term tissue culture of a functional, clonal beta (B) cell line UMR 407/3 derived from neonatal rat pancreas was described. Immunofluorescence demonstrated specific and uniform staining for insulin [9004-10-8]. TEM showed the presence of microvilli and cytoplasmic granules. The doubling time in culture was ~60 h in 2% (vol./vol.) fetal calf serum with inhibition of growth at confluence. Biochem. studies demonstrated the incorporation of [3H]leucine into proinsulin [9035-68-1] and insulin, with insulin comprising 43.6% of the total radioactivity incorporated into immune complexes. When incubated at 37° for 30 min with Krebs-Ringer bicarbonate buffer (pH 7.4), the amt. of insulin released on stimulation by 16.7 mM glucose [50-99-7], 20 mM DL-glyceraldehyde [56-82-6], or 20 mM a-ketoisocaproate [816-66-0] was significantly higher compared with that by 5.6 mM glucose. The mean insulin content was equiv. to 99 fmol/5 ′ 105 cells. Regulated insulin release was maintained through 315 passages in culture. The cells showed morphol. evidence of senescence after passage 26 and this was assocd. with significant redn. in stimulated insulin release as well as insulin content. The ability of the cells of this clonal line to grow in soft agar suggests that it is a precursor cell line. The clonal B cell lines isolated so far may thus represent variably committed rather than fully differentiated B cells in culture. These clonal non-neoplastic cell lines will be useful models with which to study the regulation of maturation/differentiation of B cells and insulin gene expression. Finally, the method described for the isolation of clonal B cell lines can be used to establish, in culture, precursor clonal lines from other normal tissues. .

Mutagenicity of and formation of oxygen radicals by trioses and glyoxal derivatives

Mutagenicity of and formation of oxygen radicals by trioses and glyoxal derivatives. Yamaguchi, Tsutomu; Nakagawa, Keiko (Dep. Food Nutr., Yamaguchi Women's Univ., Yamaguchi 753, Japan). Agric. Biol. Chem., 47(11), 2461-5 (English) 1983. CODEN: ABCHA6.Several reagents with their cas registry numbers 116-09-6 and 556-52-5 are used here. ISSN: 0002-1369. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Dihydroxyacetone [96-26-4], DL-glyceraldehyde [56-82-6], glyoxal [107-22-2], methyl glyoxal [78-98-8], and glyoxylic acid [298-12-4] were mutagenic on Salmonella typhimurium TA 100. The mutagenicities of these substances were inhibited by the addn. of S-9 or some free radical scavengers. The alk. buffered solns. of these mutagenic substances reduced Nitro Blue tetrazolium chloride [298-83-9]. DNA was degraded by the addn. of these mutagenic substances. Free radicals derived from autoxidn. of these substances are responsible for their mutagenicity. .